Distinctive Nuclear Localization Signals in the Oomycete Phytophthora sojae

Distinctive Nuclear Localization Signals in the Oomycete Phytophthora sojae

To date, nuclear localization signals (NLSs) that target proteins to nuclei in oomycetes have not been defined, but have been assumed to be the same as in higher eukaryotes. Here, we use the soybean pathogen Phytophthora sojae as a model to investigate these sequences in oomycetes. By establishing a reliable in vivo NLS assay based on confocal microscopy, we found that many canonical monopartit...

Efficient disruption and replacement of an effector gene in the oomycete Phytophthora sojae using CRISPR/Cas9.

Phytophthora sojae is an oomycete pathogen of soybean. As a result of its economic importance, P. sojae has become a model for the study of oomycete genetics, physiology and pathology. The lack of efficient techniques for targeted mutagenesis and gene replacement have long hampered genetic studies of pathogenicity in Phytophthora species. Here, we describe a CRISPR/Cas9 system enabling rapid an...

Genetic Diversity of Phytophthora Sojae in Iran

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae...

Glucan synthase of Phytophthora sojae

Genetic Diversity of Phytophthora Sojae in Iran

Objective: The aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of P. sojae from Iran. Methods: During 2005–2007, 142 isolates of Phytophthora sojae were collected from soil samples and diseased soybean plants from Lorestan, Mazandaran, Golestan and Ardabil provinces. Races of the isolates were determined. DNA of P. sojae...